- DNA structure consists of nucleotides with a phosphate, deoxyribose sugar, and a base
- Prokaryotes (bacteria) have a single circular chromosome while eukaryotes (plants, animals, fungi) have linear chromosomes in the nucleus and circular chromosomes in mitochondria and chloroplasts
- Polymerase chain reaction (PCR) is a technique used to amplify DNA with a DNA template strand, primers, free nucleotides, and a heat-tolerant DNA polymerase
- PCR technique stages include heating to break hydrogen bonds, cooling for primer annealing, and heating for DNA polymerase activation
- Gene expression involves mRNA synthesis from DNA, and translation produces a protein using the instructions from mRNA
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