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How to See Tiny Stuff: Using an Electron Microscope!

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Polar

23/10/2022

Biology

Microscopy/Magnification

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Biology

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Homeostasis and response

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Hormones in human reproduction

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Oestrogen and progesterone in the control of the menstrual cycle

How to See Tiny Stuff: Using an Electron Microscope!

A comprehensive guide to microscopy fundamentals covering Understanding electron microscope resolution, magnification calculations, and unit conversions in microscopy.

• Explores different types of microscopes including light microscopes, TEMs, and SEMs with their respective magnification capabilities
• Details the mathematical relationship between image size, actual size, and magnification
• Provides practical guidance on Converting units in microscopy measurements
• Explains How magnification affects image size calculation through worked examples
• Covers the key differences between 2D and 3D imaging capabilities across microscope types

...

23/10/2022

220

Microscopy/Magnefication Resolution: •Resolution is the shortest distance two points on a speciem.. The equation for megnefication: Image si

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Practical Applications and Calculations

The second page demonstrates practical applications of microscopy calculations through worked examples. It shows how to determine actual sizes of specimens and calculate magnification using real-world scenarios.

Example: A practical calculation shows how to find the actual size of a nucleus measuring 100mm on a diagram with ×10,000 magnification.

Highlight: The page emphasizes the importance of proper unit conversion in microscopy measurements, showing how to work between micrometers, millimeters, and meters.

Definition: The fundamental formula for magnification calculations is: Magnification = Image Size ÷ Actual Size

Example: A worked example demonstrates finding magnification when a bacterial cell appears 18mm long with an actual size of 0.002mm, resulting in ×9000 magnification.

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Subjects

Biology

Homeostasis and response

Hormones in human reproduction

Oestrogen and progesterone in the control of the menstrual cycle

 

Biology

•

220

•

23 Oct 2022

•

2 pages

How to See Tiny Stuff: Using an Electron Microscope!

user profile picture

Polar

@polar

A comprehensive guide to microscopy fundamentals covering Understanding electron microscope resolution, magnification calculations, and unit conversions in microscopy.

• Explores different types of microscopes including light microscopes, TEMs, and SEMs with their respective magnification capabilities
• Details the mathematical... Show more

Microscopy/Magnefication Resolution: •Resolution is the shortest distance two points on a speciem.. The equation for megnefication: Image si

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Practical Applications and Calculations

The second page demonstrates practical applications of microscopy calculations through worked examples. It shows how to determine actual sizes of specimens and calculate magnification using real-world scenarios.

Example: A practical calculation shows how to find the actual size of a nucleus measuring 100mm on a diagram with ×10,000 magnification.

Highlight: The page emphasizes the importance of proper unit conversion in microscopy measurements, showing how to work between micrometers, millimeters, and meters.

Definition: The fundamental formula for magnification calculations is: Magnification = Image Size ÷ Actual Size

Example: A worked example demonstrates finding magnification when a bacterial cell appears 18mm long with an actual size of 0.002mm, resulting in ×9000 magnification.

Microscopy/Magnefication Resolution: •Resolution is the shortest distance two points on a speciem.. The equation for megnefication: Image si

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Understanding Microscopy and Magnification

This comprehensive page introduces fundamental concepts in microscopy, including resolution, magnification, and different types of microscopes. The content explores how various microscopes function and their magnification capabilities.

Definition: Resolution refers to the shortest measurable distance between two points on a specimen that can be distinguished as separate entities.

Vocabulary: Magnification describes the degree to which an optical instrument can enlarge the appearance of an object.

Example: The equation for magnification is expressed as: Image size II = Actual size AA × Magnification MM

Highlight: Different microscopes offer varying levels of magnification:

  • Light Microscope: up to ×1,500
  • Transmission Electron Microscope TEMTEM: up to ×250,000
  • Scanning Electron Microscope SEMSEM: up to ×100,000

Quote: "Light microscopes only produce 2-D images, while SEMs can create 3-D images through electron reflection."

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