Light Microscopy and Key Biology Practicals- A comprehensive guide... Show more
Sign up to see the contentIt's free!
Access to all documents
Improve your grades
Join milions of students
By signing up you accept Terms of Service and Privacy Policy
152
1
Harry
12/11/2025
Biology
Biology Paper 1 - Required practicals
3,349
•
12 Nov 2025
•
GCSE Biology 1 Required Practicals: Microscopy, Osmosis, and Food Tests
1 / 5
Investigating Osmosis
This comprehensive guide covers the osmosis required practical method using potato tissue to demonstrate water movement across cell membranes.
Definition: Osmosis is the movement of water molecules from an area of higher water concentration to an area of lower water concentration across a partially permeable membrane.
Highlight: The change in potato mass or length indicates the direction of water movement - increasing in hypotonic solutions and decreasing in hypertonic solutions.
Example: A potato cylinder placed in pure water gains mass as water moves into cells, while in concentrated sugar solution it loses mass as water moves out.
Vocabulary:
- Solute: A substance that dissolves in a solvent
- Hypotonic: Solution with lower solute concentration than cell contents
- Hypertonic: Solution with higher solute concentration than cell contents
Food Tests
This section explains the food test biology practical procedures for identifying different biological molecules in food samples.
Definition: Biochemical tests use specific reagents that produce characteristic color changes to identify the presence of particular nutrients.
Highlight: Each test is qualitative, indicating only the presence or absence of the tested substance, not the quantity.
Example: Benedict's solution turns from blue to brick red when heated with reducing sugars.
Vocabulary:
- Iodine solution: Reagent that turns blue-black in presence of starch
- Benedict's solution: Reagent for testing reducing sugars
- Biuret's reagent: Test for proteins, turning purple if present
- Emulsion test: Using ethanol to identify lipids
Investigating Amylase Enzyme
This practical demonstrates how pH affects enzyme activity in the gcse biology required practical.
Definition: Amylase is an enzyme that catalyzes the breakdown of starch into simpler sugars.
Highlight: Enzyme activity is monitored by testing for the presence of starch using iodine at regular intervals.
Example: At optimal pH (7-9), starch is quickly broken down and iodine remains brown, while at extreme pH values, the enzyme denatures and starch persists.
Vocabulary:
- Enzyme: Biological catalyst that speeds up reactions
- Denatured: Loss of enzyme function due to changes in protein structure
- Optimal pH: The pH at which enzyme activity is greatest
- Activation energy: Minimum energy required for a reaction to occur
Investigating Amylase Enzyme
This practical explores enzyme activity and the effects of pH, crucial for understanding GCSE biology enzyme kinetics.
Definition: Amylase is an enzyme that catalyzes the breakdown of starch into simpler sugars.
Highlight: Enzyme activity is optimal within specific pH ranges, demonstrating the importance of environmental conditions.
Example: At pH 7-9, starch is rapidly broken down, shown by iodine remaining brown.
Vocabulary: Denaturation - the process where enzymes lose their functional shape due to environmental conditions.
Using a Light Microscope
This section details the proper use of a microscope required practical method and calculations. The practical aims to examine cellular structures and determine their sizes using microscopy techniques.
Definition: Magnification is calculated by dividing image size by actual object size, with measurements typically in micrometers (μm) or nanometers (nm).
Highlight: When using the microscope, always start with the lowest magnification lens and focus before moving to higher powers.
Example: To calculate actual size of a cell measuring 15mm in an image with 500x magnification: Actual size = 15mm ÷ 500 = 0.03mm = 30μm
Vocabulary:
- Methylene blue: A stain used to make cell structures more visible
- Resolution: The ability to distinguish between two separate points
- Magnification: The number of times larger an image appears compared to actual size
We thought you’d never ask...
What is the Knowunity AI companion?
Our AI Companion is a student-focused AI tool that offers more than just answers. Built on millions of Knowunity resources, it provides relevant information, personalised study plans, quizzes, and content directly in the chat, adapting to your individual learning journey.
Where can I download the Knowunity app?
You can download the app from Google Play Store and Apple App Store.
Is Knowunity really free of charge?
That's right! Enjoy free access to study content, connect with fellow students, and get instant help – all at your fingertips.
Can't find what you're looking for? Explore other subjects.
Students love us — and so will you.
4.9/5
App Store
4.8/5
Google Play
The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.
Stefan S
iOS user
This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.
Samantha Klich
Android user
Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.
Anna
iOS user
Best app on earth! no words because it’s too good
Thomas R
iOS user
Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.
Basil
Android user
This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.
David K
iOS user
The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!
Sudenaz Ocak
Android user
In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.
Greenlight Bonnie
Android user
very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
Rohan U
Android user
I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
iOS user
THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
iOS user
This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
iOS user
The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.
Stefan S
iOS user
This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.
Samantha Klich
Android user
Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.
Anna
iOS user
Best app on earth! no words because it’s too good
Thomas R
iOS user
Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.
Basil
Android user
This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.
David K
iOS user
The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!
Sudenaz Ocak
Android user
In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.
Greenlight Bonnie
Android user
very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
Rohan U
Android user
I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
iOS user
THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
iOS user
This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
iOS user
GCSE Biology 1 Required Practicals: Microscopy, Osmosis, and Food Tests
Light Microscopy and Key Biology Practicals - A comprehensive guide covering essential GCSE biology practical experiments including microscopy, osmosis investigation, food testing, enzyme activity, and photosynthesis studies.
• The guide details five critical required practical biologyexperiments that students commonly... Show more
Investigating Osmosis
This comprehensive guide covers the osmosis required practical method using potato tissue to demonstrate water movement across cell membranes.
Definition: Osmosis is the movement of water molecules from an area of higher water concentration to an area of lower water concentration across a partially permeable membrane.
Highlight: The change in potato mass or length indicates the direction of water movement - increasing in hypotonic solutions and decreasing in hypertonic solutions.
Example: A potato cylinder placed in pure water gains mass as water moves into cells, while in concentrated sugar solution it loses mass as water moves out.
Vocabulary:
- Solute: A substance that dissolves in a solvent
- Hypotonic: Solution with lower solute concentration than cell contents
- Hypertonic: Solution with higher solute concentration than cell contents
Sign up to see the contentIt's free!
Access to all documents
Improve your grades
Join milions of students
By signing up you accept Terms of Service and Privacy Policy
Food Tests
This section explains the food test biology practical procedures for identifying different biological molecules in food samples.
Definition: Biochemical tests use specific reagents that produce characteristic color changes to identify the presence of particular nutrients.
Highlight: Each test is qualitative, indicating only the presence or absence of the tested substance, not the quantity.
Example: Benedict's solution turns from blue to brick red when heated with reducing sugars.
Vocabulary:
- Iodine solution: Reagent that turns blue-black in presence of starch
- Benedict's solution: Reagent for testing reducing sugars
- Biuret's reagent: Test for proteins, turning purple if present
- Emulsion test: Using ethanol to identify lipids
Sign up to see the contentIt's free!
Access to all documents
Improve your grades
Join milions of students
By signing up you accept Terms of Service and Privacy Policy
Investigating Amylase Enzyme
This practical demonstrates how pH affects enzyme activity in the gcse biology required practical.
Definition: Amylase is an enzyme that catalyzes the breakdown of starch into simpler sugars.
Highlight: Enzyme activity is monitored by testing for the presence of starch using iodine at regular intervals.
Example: At optimal pH (7-9), starch is quickly broken down and iodine remains brown, while at extreme pH values, the enzyme denatures and starch persists.
Vocabulary:
- Enzyme: Biological catalyst that speeds up reactions
- Denatured: Loss of enzyme function due to changes in protein structure
- Optimal pH: The pH at which enzyme activity is greatest
- Activation energy: Minimum energy required for a reaction to occur
Sign up to see the contentIt's free!
Access to all documents
Improve your grades
Join milions of students
By signing up you accept Terms of Service and Privacy Policy
Investigating Amylase Enzyme
This practical explores enzyme activity and the effects of pH, crucial for understanding GCSE biology enzyme kinetics.
Definition: Amylase is an enzyme that catalyzes the breakdown of starch into simpler sugars.
Highlight: Enzyme activity is optimal within specific pH ranges, demonstrating the importance of environmental conditions.
Example: At pH 7-9, starch is rapidly broken down, shown by iodine remaining brown.
Vocabulary: Denaturation - the process where enzymes lose their functional shape due to environmental conditions.
Sign up to see the contentIt's free!
Access to all documents
Improve your grades
Join milions of students
By signing up you accept Terms of Service and Privacy Policy
Using a Light Microscope
This section details the proper use of a microscope required practical method and calculations. The practical aims to examine cellular structures and determine their sizes using microscopy techniques.
Definition: Magnification is calculated by dividing image size by actual object size, with measurements typically in micrometers (μm) or nanometers (nm).
Highlight: When using the microscope, always start with the lowest magnification lens and focus before moving to higher powers.
Example: To calculate actual size of a cell measuring 15mm in an image with 500x magnification: Actual size = 15mm ÷ 500 = 0.03mm = 30μm
Vocabulary:
- Methylene blue: A stain used to make cell structures more visible
- Resolution: The ability to distinguish between two separate points
- Magnification: The number of times larger an image appears compared to actual size
We thought you’d never ask...
What is the Knowunity AI companion?
Our AI Companion is a student-focused AI tool that offers more than just answers. Built on millions of Knowunity resources, it provides relevant information, personalised study plans, quizzes, and content directly in the chat, adapting to your individual learning journey.
Where can I download the Knowunity app?
You can download the app from Google Play Store and Apple App Store.
Is Knowunity really free of charge?
That's right! Enjoy free access to study content, connect with fellow students, and get instant help – all at your fingertips.
152
Smart Tools NEW
Transform this note into: ✓ 50+ Practice Questions ✓ Interactive Flashcards ✓ Full Mock Exam ✓ Essay Outlines
Mock Exam
Quiz
Flashcards
Essay
Similar content
GCSE Biology Revision Guide
Comprehensive revision guide for GCSE Biology covering key topics such as photosynthesis, enzyme function, the circulatory system, and the impact of diseases like malaria and HPV. Ideal for students preparing for exams, this resource includes explanations, data interpretation, and practical investigation methods.
Biology10
Cell Biology Essentials
Explore key concepts in cell biology, including active transport, cell specialization, and microscopy techniques. This summary covers essential processes like osmosis, diffusion, and the cell cycle, providing a comprehensive overview for AQA Combined Science students. Ideal for exam preparation and understanding fundamental biological principles.
Biology10
Osmosis Explained
Explore the process of osmosis, including the movement of water across partially permeable membranes, and the effects on plant and animal cells in different solutions. This summary covers key concepts such as hypertonic and hypotonic solutions, plasmolysis, and the structural differences between plant and animal cells. Ideal for biology students preparing for exams.
Biology8
Biology Required Practicals Overview
Explore the essential first four required practicals for GCSE Biology, including microscopy techniques, microbiology methods, osmosis experiments, and food testing protocols. This summary provides clear steps and safety guidelines to enhance your understanding and preparation for practical assessments.
Biology10
Essential Biology Practicals
Explore key practicals for GCSE AQA Biology Paper 1, including microscopy techniques, the effects of light on photosynthesis, food tests for biomolecules, enzyme activity, and osmosis in plant tissues. This summary provides essential insights and methods for conducting experiments effectively, tailored for Higher GCSE combined science students.
Biology9
Biology Paper 1 Overview
Comprehensive study notes covering key concepts in cellular biology, human digestion, respiration, photosynthesis, and the circulatory system. This resource includes detailed explanations of cell structures, enzyme functions, nutrient absorption, and the impact of environmental factors on biological processes. Ideal for students preparing for Biology Paper 1 exams.
Biology11
Can't find what you're looking for? Explore other subjects.
Students love us — and so will you.
4.9/5
App Store
4.8/5
Google Play
The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.
Stefan S
iOS user
This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.
Samantha Klich
Android user
Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.
Anna
iOS user
Best app on earth! no words because it’s too good
Thomas R
iOS user
Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.
Basil
Android user
This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.
David K
iOS user
The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!
Sudenaz Ocak
Android user
In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.
Greenlight Bonnie
Android user
very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
Rohan U
Android user
I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
iOS user
THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
iOS user
This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
iOS user
The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.
Stefan S
iOS user
This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.
Samantha Klich
Android user
Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.
Anna
iOS user
Best app on earth! no words because it’s too good
Thomas R
iOS user
Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.
Basil
Android user
This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.
David K
iOS user
The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!
Sudenaz Ocak
Android user
In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.
Greenlight Bonnie
Android user
very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
Rohan U
Android user
I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
iOS user
THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
iOS user
This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
iOS user