This practical guide explores how to measure membrane permeability using... Show more
Biology84 views·Updated May 13, 2026·2 pagesInvestigating Membrane Permeability: Practical Method and Risks
Ellie Wood@elliewood_vugw
1
of 2
Measuring Membrane Permeability
Beetroot cells contain a purple pigment called betalain that leaks out when the cell membrane becomes more permeable. This makes beetroot an excellent tool for investigating factors that affect membrane permeability.
To set up this experiment, you'll need to cut beetroot into identical cubes, clean off any initially released pigment, and then subject these cubes to different conditions. If testing temperature effects, place each cube in distilled water at different temperatures . For solvent concentration tests, create a dilution series of ethanol (0%-100%) in distilled water.
After leaving the beetroot cubes in solution for about 20 minutes, filter the samples and measure the amount of pigment released using a colorimeter with a blue filter. The higher the absorbance reading, the more pigment has leaked out, indicating greater membrane permeability.
Quick Tip: The more pigment you can see in the water, the more permeable the membrane has become. This visual indicator makes it easy to see results even before using the colorimeter!
2
of 2
Risk Assessment
When conducting this practical, be aware of several important safety considerations. When using a scalpel to cut the beetroot, always cut away from your fingers and keep the scalpel away from the edge of your desk to prevent accidental cuts.
Glass equipment presents a risk of breakage. Handle all glassware with care and clean up any broken glass promptly and safely. If an accident occurs, don't remove embedded glass—instead, elevate the area, apply pressure, and seek help.
Ethanol used in the experiment is both an irritant and flammable. Always wear eye protection and keep ethanol away from naked flames. For hot liquids in water baths, use tongs to handle test tubes and wear appropriate eye protection to prevent scalding.
Safety First: Most lab accidents happen when students rush or get distracted. Take your time and stay focused on each step of the procedure to keep yourself and your classmates safe.
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Biology84 views·Updated May 13, 2026·2 pagesInvestigating Membrane Permeability: Practical Method and Risks
Ellie Wood@elliewood_vugw
This practical guide explores how to measure membrane permeability using beetroot cells. You'll learn how to conduct a simple experiment that shows how different conditions affect how easily substances pass through cell membranes.
1
of 2Sign up to see the content. It's free!
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Measuring Membrane Permeability
Beetroot cells contain a purple pigment called betalain that leaks out when the cell membrane becomes more permeable. This makes beetroot an excellent tool for investigating factors that affect membrane permeability.
To set up this experiment, you'll need to cut beetroot into identical cubes, clean off any initially released pigment, and then subject these cubes to different conditions. If testing temperature effects, place each cube in distilled water at different temperatures . For solvent concentration tests, create a dilution series of ethanol (0%-100%) in distilled water.
After leaving the beetroot cubes in solution for about 20 minutes, filter the samples and measure the amount of pigment released using a colorimeter with a blue filter. The higher the absorbance reading, the more pigment has leaked out, indicating greater membrane permeability.
Quick Tip: The more pigment you can see in the water, the more permeable the membrane has become. This visual indicator makes it easy to see results even before using the colorimeter!
2
of 2Sign up to see the content. It's free!
- Access to all documents
- Improve your grades
- Join milions of students
Risk Assessment
When conducting this practical, be aware of several important safety considerations. When using a scalpel to cut the beetroot, always cut away from your fingers and keep the scalpel away from the edge of your desk to prevent accidental cuts.
Glass equipment presents a risk of breakage. Handle all glassware with care and clean up any broken glass promptly and safely. If an accident occurs, don't remove embedded glass—instead, elevate the area, apply pressure, and seek help.
Ethanol used in the experiment is both an irritant and flammable. Always wear eye protection and keep ethanol away from naked flames. For hot liquids in water baths, use tongs to handle test tubes and wear appropriate eye protection to prevent scalding.
Safety First: Most lab accidents happen when students rush or get distracted. Take your time and stay focused on each step of the procedure to keep yourself and your classmates safe.
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What is the Knowunity AI companion?
Our AI Companion is a student-focused AI tool that offers more than just answers. Built on millions of Knowunity resources, it provides relevant information, personalised study plans, quizzes, and content directly in the chat, adapting to your individual learning journey.
Where can I download the Knowunity app?
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Is Knowunity really free of charge?
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