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2 Jan 2026

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5 pages

Understanding Photosynthesis: Light-Dependent and Light-Independent Reactions

sophie

@sophie_tilley

Photosynthesis is how plants make their own food using light,... Show more

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$Photosynemesis overall equation $6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2$ light dependent stage The photolysis of water releases e$

The Basics of Photosynthesis

Think of photosynthesis as nature's solar panel system - plants convert sunlight into chemical energy that powers pretty much everything on Earth. The overall equation is straightforward: 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂, meaning carbon dioxide plus water creates glucose plus oxygen.

The magic happens in chloroplasts, which are mainly found in the palisade mesophyll cells of leaves. These tiny green structures contain thylakoids - flattened sacs that stack up like pancakes to form grana, where the light-dependent reactions occur.

Leaves are perfectly designed for their job. They're thin so light penetrates through, have a large surface area to capture maximum light, and stomatal pores allow CO₂ to diffuse in. The waxy cuticle and transparent epidermis don't block light from reaching the chloroplasts.

Key Point: Chloroplasts are transducers - they change light energy into chemical energy, just like how solar panels convert sunlight into electricity!

Different photosynthetic pigments absorb different wavelengths of light. Chlorophyll A (the main one) absorbs red and blue light, chlorophyll B helps capture more blue-green light, and carotenoids like β-carotene grab the yellow-orange wavelengths that chlorophyll misses.

$Photosynemesis overall equation $6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2$ light dependent stage The photolysis of water releases e$

Light-Dependent Reactions and Photosystems

The light-dependent stage is where the real action starts - it's like the power station of photosynthesis. Photosystems are collections of pigment molecules that work together like satellite dishes, capturing light energy and funnelling it to reaction centres containing chlorophyll A.

There are two main photosystems: PSI (absorbs light at 700nm) and PSII (absorbs at 680nm). They work together in what's called non-cyclic photophosphorylation - think of it as an assembly line where electrons get passed along an electron transport chain.

Photolysis is the splitting of water molecules, which provides electrons to replace those lost from PSII, releases protons that help make ATP, and produces oxygen as a bonus waste product. The energy from moving electrons pumps protons across the thylakoid membrane, creating a concentration gradient.

Remember: The light-dependent reactions produce ATP and reduced NADP - these are like recharged batteries that power the next stage!

Chemiosmosis is how ATP gets made. Protons flow back through ATP synthase (like water through a turbine), providing energy to stick phosphate onto ADP. Meanwhile, electrons from PSI combine with NADP and protons to make reduced NADP.

$Photosynemesis overall equation $6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2$ light dependent stage The photolysis of water releases e$

The Calvin Cycle (Light-Independent Reactions)

The Calvin cycle happens in the stroma and doesn't directly need light - but it absolutely depends on the ATP and reduced NADP from the light-dependent stage. This is where CO₂ actually gets turned into glucose through a clever recycling process.

Carbon fixation starts when CO₂ combines with a 5-carbon sugar called ribulose bisphosphate (RuBP) using the enzyme rubisco. This creates an unstable 6-carbon compound that immediately splits into two molecules of glycerate-3-phosphate (GP).

The reduction phase uses energy from ATP and electrons from reduced NADP to convert GP into triose phosphate (TP) - this is the first actual sugar made in photosynthesis! Most of the TP gets recycled back to regenerate RuBP (using more ATP), but some can be converted into glucose, starch, or other carbohydrates.

Fun Fact: For every 6 molecules of TP made, only 1 becomes glucose while 5 get recycled - it's an efficient but resource-intensive process!

The cycle needs constant inputs: 3 CO₂ molecules, 9 ATP, and 6 reduced NADP to make one molecule of TP. Without the light-dependent reactions providing fresh ATP and reduced NADP, the whole system grinds to a halt.

$Photosynemesis overall equation $6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2$ light dependent stage The photolysis of water releases e$

Limiting Factors in Photosynthesis

Limiting factors control how fast photosynthesis can happen - think of them as bottlenecks in the system. The main ones are light intensity, temperature, and CO₂ concentration, and whichever is in shortest supply becomes the limiting factor.

Light intensity affects the rate up to a point called light saturation, where the light-dependent reactions can't work any faster. Beyond this point, something else becomes limiting. The light compensation point is where photosynthesis exactly balances respiration.

Temperature affects photosynthesis because it's controlled by enzymes like rubisco. Higher temperatures increase reaction rates (more kinetic energy), but too much heat denatures the enzymes. This is why you see different optimal temperatures for different plants.

CO₂ concentration directly affects the Calvin cycle since it's a raw material. In normal air (about 0.04% CO₂), this is often the limiting factor. Increasing CO₂ boosts photosynthesis until something else becomes limiting.

Pro Tip: Understanding limiting factors helps explain why commercial greenhouses control temperature, light, and CO₂ levels to maximise plant growth!

Mineral nutrition also matters. Plants need magnesium for chlorophyll (deficiency causes yellowing called chlorosis), nitrogen for amino acids and proteins, and phosphate for ATP and NADP.

$Photosynemesis overall equation $6CO_2 + 6H_2O \rightarrow C_6H_{12}O_6 + 6O_2$ light dependent stage The photolysis of water releases e$

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115

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2 Jan 2026

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5 pages