Understanding Light and Electron Microscopes
Light microscopes have been around since the 16th century and work by passing light through your specimen to create a magnified image using lenses. They're perfect for observing basic cell structures, but they can't show you the detailed sub-cellular structures inside cells.
Electron microscopes, first used in 1933, are the superstars of the microscopy world. Instead of light, they fire electrons at your specimen, giving you incredible detail of sub-cellular structures that light microscopes simply can't reveal.
Setting up a light microscope experiment is straightforward: place your thin tissue sample on a slide, add a few drops of iodine stain, cover with a coverslip, and start with the lowest magnification before working your way up.
Top Tip: Always start with low magnification to locate your specimen, then gradually increase to see finer details!
Understanding metric prefixes is crucial for microscopy measurements. From kilometres (10³m) down to picometres (10⁻¹²m), these units help you describe everything from the width of a hair to the size of molecules.