The Krebs Cycle and Oxidative Phosphorylation
The Krebs cycle is like a recycling plant in your mitochondrial matrix that squeezes every bit of energy from acetyl CoA. It's a circular process that regenerates oxaloacetate to keep the cycle spinning.
Starting with acetyl CoA combining with oxaloacetate to form citrate, the cycle involves two decarboxylation steps (removing CO₂) and multiple dehydrogenation reactions. Each turn produces 1 ATP, 3 reduced NAD, and 1 reduced FAD. Since it runs twice per glucose molecule, you get double these amounts.
Oxidative phosphorylation is where the real energy jackpot happens. In the inner mitochondrial membrane, reduced NAD and FAD release hydrogen, which splits into protons and electrons. The electrons travel down the electron transport chain, losing energy that's used to pump protons into the intermembrane space.
This creates an electrochemical gradient that drives chemiosmosis - protons flow back through ATP synthase, producing ATP. Oxygen acts as the final electron acceptor, combining with protons and electrons to form water. The total yield? Up to 32 ATP molecules from one glucose!
Key Point: Oxygen is essential here - without it, the electron transport chain stops, and ATP production plummets.