Subjects

Careers

Open the App

Subjects

728

โ€ข

22 Dec 2022

โ€ข

1 page

AQA A-Level Biology: ELISA Test Revision Notes - Free PDF Download

The ELISA test(enzyme-linked immunosorbent assay) is a crucial diagnostic... Show more


<p>ELISA stands for enzyme-linked immunosorbent assay.</p>
<p>ELISA tests can be used to detect the presence of antibodies to a specific an

ELISA Test Fundamentals and Applications

The first page introduces the fundamental concepts of ELISA test methodology and its practical applications in medical diagnostics. The text explains how this immunological technique works through specific antibody-antigen interactions and subsequent enzymatic reactions.

Definition: ELISA enzymeโˆ’linkedimmunosorbentassayenzyme-linked immunosorbent assay is a diagnostic test that uses enzyme-linked antibodies to detect specific antigens or antibodies in patient samples.

Highlight: The test's reliability comes from its specific washing steps that prevent false positive results by removing unbound antibodies.

Example: In HIV testing, HIV antigens are fixed to the reaction vessel, and patient blood plasma is tested for HIV-specific antibodies. A positive result is indicated by a color change after adding the enzyme substrate.

Vocabulary: PSA Prostateโˆ’SpecificAntigenProstate-Specific Antigen - A protein marker used to screen for prostate cancer through direct ELISA procedure.

The page details two specific examples of indirect ELISA test applications:

  1. HIV Testing Protocol:
  • Involves binding HIV antigens to the reaction vessel
  • Uses primary antibodies from patient samples
  • Employs enzyme-linked secondary antibodies
  • Includes crucial washing steps
  • Results in color change for positive samples
  1. Prostate Cancer Screening:
  • Tests for elevated PSA levels in blood plasma
  • Uses antibodies specific to PSA
  • Employs a similar indirect ELISA methodology
  • Helps identify potential prostate cancer cases for further investigation

Highlight: The types of ELISA discussed include both direct and indirect methods, with indirect ELISA offering enhanced sensitivity through its two-antibody approach.



Can't find what you're looking for? Explore other subjects.

Students love us โ€” and so will you.

4.9/5

App Store

4.8/5

Google Play

The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because itโ€™s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didnโ€™t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as Iโ€™m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH ๐Ÿ˜๐Ÿ˜๐Ÿ˜ฒ๐Ÿค‘๐Ÿ’—โœจ๐ŸŽ€๐Ÿ˜ฎ

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user

The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because itโ€™s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didnโ€™t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as Iโ€™m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH ๐Ÿ˜๐Ÿ˜๐Ÿ˜ฒ๐Ÿค‘๐Ÿ’—โœจ๐ŸŽ€๐Ÿ˜ฎ

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user

ย 

Biology

โ€ข

728

โ€ข

22 Dec 2022

โ€ข

1 page

AQA A-Level Biology: ELISA Test Revision Notes - Free PDF Download

The ELISA test (enzyme-linked immunosorbent assay) is a crucial diagnostic tool in AQA A Level Biologythat detects specific antibodies or antigens in patient samples. This versatile test employs enzyme-linked antibodies that produce color changes to indicate the presence of... Show more


<p>ELISA stands for enzyme-linked immunosorbent assay.</p>
<p>ELISA tests can be used to detect the presence of antibodies to a specific an

Sign up to see the contentIt's free!

Access to all documents

Improve your grades

Join milions of students

By signing up you accept Terms of Service and Privacy Policy

ELISA Test Fundamentals and Applications

The first page introduces the fundamental concepts of ELISA test methodology and its practical applications in medical diagnostics. The text explains how this immunological technique works through specific antibody-antigen interactions and subsequent enzymatic reactions.

Definition: ELISA enzymeโˆ’linkedimmunosorbentassayenzyme-linked immunosorbent assay is a diagnostic test that uses enzyme-linked antibodies to detect specific antigens or antibodies in patient samples.

Highlight: The test's reliability comes from its specific washing steps that prevent false positive results by removing unbound antibodies.

Example: In HIV testing, HIV antigens are fixed to the reaction vessel, and patient blood plasma is tested for HIV-specific antibodies. A positive result is indicated by a color change after adding the enzyme substrate.

Vocabulary: PSA Prostateโˆ’SpecificAntigenProstate-Specific Antigen - A protein marker used to screen for prostate cancer through direct ELISA procedure.

The page details two specific examples of indirect ELISA test applications:

  1. HIV Testing Protocol:
  • Involves binding HIV antigens to the reaction vessel
  • Uses primary antibodies from patient samples
  • Employs enzyme-linked secondary antibodies
  • Includes crucial washing steps
  • Results in color change for positive samples
  1. Prostate Cancer Screening:
  • Tests for elevated PSA levels in blood plasma
  • Uses antibodies specific to PSA
  • Employs a similar indirect ELISA methodology
  • Helps identify potential prostate cancer cases for further investigation

Highlight: The types of ELISA discussed include both direct and indirect methods, with indirect ELISA offering enhanced sensitivity through its two-antibody approach.

Can't find what you're looking for? Explore other subjects.

Students love us โ€” and so will you.

4.9/5

App Store

4.8/5

Google Play

The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because itโ€™s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didnโ€™t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as Iโ€™m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH ๐Ÿ˜๐Ÿ˜๐Ÿ˜ฒ๐Ÿค‘๐Ÿ’—โœจ๐ŸŽ€๐Ÿ˜ฎ

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user

The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because itโ€™s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didnโ€™t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as Iโ€™m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH ๐Ÿ˜๐Ÿ˜๐Ÿ˜ฒ๐Ÿค‘๐Ÿ’—โœจ๐ŸŽ€๐Ÿ˜ฎ

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user