Izražanje genov je temeljni proces, ki omogoča celicam, da iz... Show more
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Updated May 20, 2026
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Osnove izražanja genov in sinteza proteinov
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Uvod v izražanje genov
Ko se vprašaš, kako celica "ve", kdaj mora narediti določen protein, je odgovor v izražanju genov. To je proces, kjer se informacija iz DNA pretvori v funkcionalen produkt - običajno protein.
Gen je osnovna enota dedovanja - odsek DNA, ki nosi navodila za tvorbo določenega proteina. Celoten proces poteka v dveh glavnih korakih: transkripcija (prepisovanje DNA v mRNA) in translacija (prevajanje mRNA v protein).
Ključne molekule, ki jih moraš poznati, so mRNA (prenašalka informacij), tRNA (prinaša aminokisline) in rRNA (sestavni del ribosomov). RNA-polimeraza je encim, ki piše mRNA po DNA predlogi, kodon pa je trojica nukleotidov, ki določa, katera aminokislina pride na vrsto.
💡 Zapomni si: Genetski kod deluje kot slovar - vsaka trojica črk (kodon) pomeni eno aminokislino!
Transkripcija - prepisovanje genov
Transkripcija je prvi korak, kjer RNA-polimeraza "prebere" DNA in napiše komplementarno mRNA kopijo. Pri evkariontih poteka v jedru, pri prokariontih pa v citoplazmi.
Proces se začne z iniciacijo, kjer se RNA-polimeraza veže na promotor - "start" signal na DNA. Dvojna vijačnica se razvije in encim začne svojo nalogo. Sledi elongacija, kjer se RNA-polimeraza premika po matrični verigi DNA in dodaja ribonukleotide v smeri 5' → 3'. Pomembno: namesto timina (T) uporablja uracil (U)!
Terminacija nastopi, ko encim doseže terminator - "stop" signal. Pri evkariontih sledi še post-transkripcijska obdelava: izrezovanje intronov, dodajanje 5' kape in 3' poli-A repa. Šele tako nastane zrela mRNA, pripravljena za transport v citoplazmo.
💡 Pametno: Kodirajoča veriga DNA ima enako zaporedje kot mRNA (le T namesto U) - uporabi to za preverjanje!
Obdelava mRNA pri evkariontih
Sveže napisana pre-mRNA še ni pripravljena za uporabo - potrebuje "urejanje". Ta proces vključuje tri ključne korake, ki so edinstveni za evkarionte.
Prvo je izrezovanje intronov (splicing), kjer se nekodirajoči odseki (introni) odstranijo, kodirajoči odseki (eksoni) pa se spojijo. Ta proces omogoča alternativno izrezovanje - iz istega gena lahko nastanejo različni proteini!
Dodajanje 5' kape ščiti mRNA pred razgradnjo in omogoča vezavo na ribosom. 3' poli-A rep dodatno stabilizira molekulo in olajša transport iz jedra. Šele po vseh teh spremembah je mRNA zrela za translacijo.
💡 Razlika: Prokarionti nimajo intronov, zato njihova mRNA ne potrebuje obdelave!
Genetski kod in priprava na translacijo
Genetski kod je univerzalni "slovar" življenja, ki velja od bakterij do človeka. Je tripleten , nedvoumen (vsak kodon pomeni le eno aminokislino) in degeneriran (večina aminokislin ima več kodonov).
Pred translacijo se mora vsaka aminokislina vezati na svojo tRNA molekulo. To reakcijo katalizira aminoacil-tRNA-sintetaza in porabi ATP. Vsaka tRNA ima antikodon, ki se pari s komplementarnim kodonom na mRNA.
Start kodon AUG kodira metionin in začne translacijo, stop kodoni (UAA, UAG, UGA) pa je končajo. Ta sistem omogoča natančno "branje" genetskih navodil in sintezo pravilnih proteinov.
💡 Zapomniti: Če se kodon spremeni zaradi mutacije, se lahko spremeni celoten protein!
Translacija - sinteza proteinov
Translacija poteka na ribosomih v citoplazmi in vključuje štiri glavne faze. Najprej se v iniciaciji mala ribosomska podenota veže na mRNA in najde start kodon AUG. Pridruži se velika podenota in nastane funkcionalen ribosom.
Elongacija je srce procesa: na A-mesto ribosoma vstopi tRNA z ustreznim antikodonom, rRNA katalizira nastanek peptidne vezi, ribosom se pomakne za en kodon naprej (translokacija). "Izrabljena" tRNA zapusti ribosom preko E-mesta.
Terminacija nastopi pri stop kodonu, kjer se veže sprostitveni faktor. Polipeptidna veriga se sprosti in celoten kompleks razpade. Tako nastane nov protein, pripravljen za svojo nalogo v celici.
💡 Predstavljaj si: Ribosom je kot tovarna, ki "bere" navodila (mRNA) in sestavlja produkt (protein) iz surovin (aminokislin)!
Praktični primeri in mutacije
Poglejmo konkreten primer: DNA zaporedje 3'-TAC CGT ACT-5' (matrična veriga) da mRNA 5'-AUG GCA UGA-3'. To se prevede v Met-Ala, ker je UGA stop kodon.
Točkovne mutacije lahko drastično spremenijo protein. Pri srpastocelični anemiji se en sam nukleotid spremeni: GAG (glutaminska kislina) → GUG (valin). Ker je glutaminska kislina hidrofilna, valin pa hidrofoben, se hemoglobin deformira in povzroči bolezen.
Ta primer kaže, kako kritično je natančno "branje" genetskega koda. Ena napaka lahko povzroči resne posledice, zato imajo celice različne mehanizme za preverjanje in popravljanje napak.
💡 Ključno spoznanje: Genetski kod je tako občutljiv, da lahko ena spremenjena črka povzroči resno bolezen!
Razlike med prokarionti in evkarionti
Prokarionti imajo sklopljeno transkripcijo in translacijo - oba procesa potekata sočasno v citoplazmi. Njihovi geni večinoma nimajo intronov, zato mRNA ne potrebuje obdelave.
Evkarionti imajo procesa ločena: transkripcija v jedru, translacija v citoplazmi. Jedrna ovojnica omogoča post-transkripcijsko obdelavo mRNA. Njihovi geni vsebujejo introne in eksone, kar omogoča alternativno izrezovanje.
Ta razlika je ključna za razumevanje evolucije - evkarionti lahko iz istega gena naredijo več različnih proteinov, kar povečuje njihovo prilagodljivost. Prokarionti so enostavnejši, a zato hitrejši pri odzivanju na spremembe.
💡 Za maturo: Ta tabela je pogosto vprašanje - obvladaj razlike med tema dvema skupinama!
Hiter povzetek za ponavljanje
Celoten proces: DNA → (transkripcija v jedru) → pre-mRNA → (obdelava) → zrela mRNA → (transport) → (translacija na ribosomih) → Protein.
Transkripcija: RNA-polimeraza + DNA matrica = mRNA. Faze: iniciacija (promotor), elongacija (5'→3'), terminacija (terminator). Translacija: ribosom + mRNA + tRNA = protein. Kodoni določajo aminokisline, start je AUG, stop so UAA/UAG/UGA.
Genetski kod je univerzalen, tripleten in degeneriran. Ključne molekule: DNA (shramba), mRNA (glasnik), tRNA (dostavljavec), rRNA (katalizator). Pri evkariontih dodaj še obdelavo mRNA!
💡 Zadnji nasvet: Vadi s konkretnimi zaporedji - pretvarjanje DNA→mRNA→protein je pogosto na testih!
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Where can I download the Knowunity app?
You can download the app from Google Play Store and Apple App Store.
Is Knowunity really free of charge?
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very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
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I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
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THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE Knowunity AI. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
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This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
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The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.
Stefan S
iOS user
This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.
Samantha Klich
Android user
Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.
Anna
iOS user
Best app on earth! no words because it’s too good
Thomas R
iOS user
Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.
Basil
Android user
This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.
David K
iOS user
The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!
Sudenaz Ocak
Android user
In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.
Greenlight Bonnie
Android user
very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
Rohan U
Android user
I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
iOS user
THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE Knowunity AI. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
iOS user
This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
iOS user
Osnove izražanja genov in sinteza proteinov
Izražanje genov je temeljni proces, ki omogoča celicam, da iz genetskih informacij v DNA proizvajajo potrebne proteine. Celoten proces sledi centralni dogmi molekularne biologije: DNA → RNA → Protein.
Uvod v izražanje genov
Ko se vprašaš, kako celica "ve", kdaj mora narediti določen protein, je odgovor v izražanju genov. To je proces, kjer se informacija iz DNA pretvori v funkcionalen produkt - običajno protein.
Gen je osnovna enota dedovanja - odsek DNA, ki nosi navodila za tvorbo določenega proteina. Celoten proces poteka v dveh glavnih korakih: transkripcija (prepisovanje DNA v mRNA) in translacija (prevajanje mRNA v protein).
Ključne molekule, ki jih moraš poznati, so mRNA (prenašalka informacij), tRNA (prinaša aminokisline) in rRNA (sestavni del ribosomov). RNA-polimeraza je encim, ki piše mRNA po DNA predlogi, kodon pa je trojica nukleotidov, ki določa, katera aminokislina pride na vrsto.
💡 Zapomni si: Genetski kod deluje kot slovar - vsaka trojica črk (kodon) pomeni eno aminokislino!
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Transkripcija - prepisovanje genov
Transkripcija je prvi korak, kjer RNA-polimeraza "prebere" DNA in napiše komplementarno mRNA kopijo. Pri evkariontih poteka v jedru, pri prokariontih pa v citoplazmi.
Proces se začne z iniciacijo, kjer se RNA-polimeraza veže na promotor - "start" signal na DNA. Dvojna vijačnica se razvije in encim začne svojo nalogo. Sledi elongacija, kjer se RNA-polimeraza premika po matrični verigi DNA in dodaja ribonukleotide v smeri 5' → 3'. Pomembno: namesto timina (T) uporablja uracil (U)!
Terminacija nastopi, ko encim doseže terminator - "stop" signal. Pri evkariontih sledi še post-transkripcijska obdelava: izrezovanje intronov, dodajanje 5' kape in 3' poli-A repa. Šele tako nastane zrela mRNA, pripravljena za transport v citoplazmo.
💡 Pametno: Kodirajoča veriga DNA ima enako zaporedje kot mRNA (le T namesto U) - uporabi to za preverjanje!
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Obdelava mRNA pri evkariontih
Sveže napisana pre-mRNA še ni pripravljena za uporabo - potrebuje "urejanje". Ta proces vključuje tri ključne korake, ki so edinstveni za evkarionte.
Prvo je izrezovanje intronov (splicing), kjer se nekodirajoči odseki (introni) odstranijo, kodirajoči odseki (eksoni) pa se spojijo. Ta proces omogoča alternativno izrezovanje - iz istega gena lahko nastanejo različni proteini!
Dodajanje 5' kape ščiti mRNA pred razgradnjo in omogoča vezavo na ribosom. 3' poli-A rep dodatno stabilizira molekulo in olajša transport iz jedra. Šele po vseh teh spremembah je mRNA zrela za translacijo.
💡 Razlika: Prokarionti nimajo intronov, zato njihova mRNA ne potrebuje obdelave!
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Genetski kod in priprava na translacijo
Genetski kod je univerzalni "slovar" življenja, ki velja od bakterij do človeka. Je tripleten , nedvoumen (vsak kodon pomeni le eno aminokislino) in degeneriran (večina aminokislin ima več kodonov).
Pred translacijo se mora vsaka aminokislina vezati na svojo tRNA molekulo. To reakcijo katalizira aminoacil-tRNA-sintetaza in porabi ATP. Vsaka tRNA ima antikodon, ki se pari s komplementarnim kodonom na mRNA.
Start kodon AUG kodira metionin in začne translacijo, stop kodoni (UAA, UAG, UGA) pa je končajo. Ta sistem omogoča natančno "branje" genetskih navodil in sintezo pravilnih proteinov.
💡 Zapomniti: Če se kodon spremeni zaradi mutacije, se lahko spremeni celoten protein!
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Translacija - sinteza proteinov
Translacija poteka na ribosomih v citoplazmi in vključuje štiri glavne faze. Najprej se v iniciaciji mala ribosomska podenota veže na mRNA in najde start kodon AUG. Pridruži se velika podenota in nastane funkcionalen ribosom.
Elongacija je srce procesa: na A-mesto ribosoma vstopi tRNA z ustreznim antikodonom, rRNA katalizira nastanek peptidne vezi, ribosom se pomakne za en kodon naprej (translokacija). "Izrabljena" tRNA zapusti ribosom preko E-mesta.
Terminacija nastopi pri stop kodonu, kjer se veže sprostitveni faktor. Polipeptidna veriga se sprosti in celoten kompleks razpade. Tako nastane nov protein, pripravljen za svojo nalogo v celici.
💡 Predstavljaj si: Ribosom je kot tovarna, ki "bere" navodila (mRNA) in sestavlja produkt (protein) iz surovin (aminokislin)!
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Praktični primeri in mutacije
Poglejmo konkreten primer: DNA zaporedje 3'-TAC CGT ACT-5' (matrična veriga) da mRNA 5'-AUG GCA UGA-3'. To se prevede v Met-Ala, ker je UGA stop kodon.
Točkovne mutacije lahko drastično spremenijo protein. Pri srpastocelični anemiji se en sam nukleotid spremeni: GAG (glutaminska kislina) → GUG (valin). Ker je glutaminska kislina hidrofilna, valin pa hidrofoben, se hemoglobin deformira in povzroči bolezen.
Ta primer kaže, kako kritično je natančno "branje" genetskega koda. Ena napaka lahko povzroči resne posledice, zato imajo celice različne mehanizme za preverjanje in popravljanje napak.
💡 Ključno spoznanje: Genetski kod je tako občutljiv, da lahko ena spremenjena črka povzroči resno bolezen!
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Razlike med prokarionti in evkarionti
Prokarionti imajo sklopljeno transkripcijo in translacijo - oba procesa potekata sočasno v citoplazmi. Njihovi geni večinoma nimajo intronov, zato mRNA ne potrebuje obdelave.
Evkarionti imajo procesa ločena: transkripcija v jedru, translacija v citoplazmi. Jedrna ovojnica omogoča post-transkripcijsko obdelavo mRNA. Njihovi geni vsebujejo introne in eksone, kar omogoča alternativno izrezovanje.
Ta razlika je ključna za razumevanje evolucije - evkarionti lahko iz istega gena naredijo več različnih proteinov, kar povečuje njihovo prilagodljivost. Prokarionti so enostavnejši, a zato hitrejši pri odzivanju na spremembe.
💡 Za maturo: Ta tabela je pogosto vprašanje - obvladaj razlike med tema dvema skupinama!
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Improve your grades
Join milions of students
Hiter povzetek za ponavljanje
Celoten proces: DNA → (transkripcija v jedru) → pre-mRNA → (obdelava) → zrela mRNA → (transport) → (translacija na ribosomih) → Protein.
Transkripcija: RNA-polimeraza + DNA matrica = mRNA. Faze: iniciacija (promotor), elongacija (5'→3'), terminacija (terminator). Translacija: ribosom + mRNA + tRNA = protein. Kodoni določajo aminokisline, start je AUG, stop so UAA/UAG/UGA.
Genetski kod je univerzalen, tripleten in degeneriran. Ključne molekule: DNA (shramba), mRNA (glasnik), tRNA (dostavljavec), rRNA (katalizator). Pri evkariontih dodaj še obdelavo mRNA!
💡 Zadnji nasvet: Vadi s konkretnimi zaporedji - pretvarjanje DNA→mRNA→protein je pogosto na testih!
We thought you’d never ask...
What is the Knowunity AI companion?
Our AI Companion is a student-focused AI tool that offers more than just answers. Built on millions of Knowunity resources, it provides relevant information, personalised study plans, quizzes, and content directly in the chat, adapting to your individual learning journey.
Where can I download the Knowunity app?
You can download the app from Google Play Store and Apple App Store.
Is Knowunity really free of charge?
That's right! Enjoy free access to study content, connect with fellow students, and get instant help – all at your fingertips.
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Students love us — and so will you.
4.6/5
App Store
4.7/5
Google Play
The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.
Stefan S
iOS user
This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.
Samantha Klich
Android user
Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.
Anna
iOS user
Best app on earth! no words because it’s too good
Thomas R
iOS user
Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.
Basil
Android user
This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.
David K
iOS user
The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!
Sudenaz Ocak
Android user
In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.
Greenlight Bonnie
Android user
very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
Rohan U
Android user
I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
iOS user
THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE Knowunity AI. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
iOS user
This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
iOS user
The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.
Stefan S
iOS user
This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.
Samantha Klich
Android user
Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.
Anna
iOS user
Best app on earth! no words because it’s too good
Thomas R
iOS user
Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.
Basil
Android user
This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.
David K
iOS user
The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!
Sudenaz Ocak
Android user
In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.
Greenlight Bonnie
Android user
very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.
Rohan U
Android user
I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.
Xander S
iOS user
THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE Knowunity AI. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮
Elisha
iOS user
This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now
Paul T
iOS user