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Fun Steps in Chromatography: TLC and Gas Chromatography for Kids

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Fun Steps in Chromatography: TLC and Gas Chromatography for Kids
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Aasiyah Rahman

@aasiyahrahman

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44 Followers

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Chromatography is a powerful technique for separating mixtures into their components. It relies on differences in how substances interact with a stationary phase and mobile phase. Thin layer chromatography separation process, gas-liquid chromatography retention time, and column chromatography setup instructions are key concepts in this field. The main types are thin-layer chromatography (TLC), column chromatography (CC), and gas-liquid chromatography (GLC), each with unique applications and procedures.

Key points:
• Chromatography separates mixtures based on component interactions with stationary and mobile phases
• TLC uses a solid silica plate and is useful for analyzing small samples
• Column chromatography employs a vertical tube filled with stationary phase material
• GLC is ideal for volatile compounds and uses retention time for identification
• Each method has specific setup procedures and analysis techniques

05/04/2023

91

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

View

Thin-Layer Chromatography (TLC)

Thin-layer chromatography (TLC) is a simple yet powerful technique for analyzing small samples through separation. This section covers the thin layer chromatography procedure and principles in detail.

The TLC stationary phase typically consists of a thin metal sheet coated with alumina (Al2O3) or silica (SiO2). Solute molecules adsorb onto this surface, with more strongly interacting components sticking more tightly. The mobile phase, usually a liquid solvent, flows over the stationary phase carrying the sample components.

To conduct a TLC analysis:

  1. Prepare a small volume of solute dissolved in solvent
  2. Draw a horizontal baseline in pencil near the bottom edge of the TLC plate
  3. Spot pure reference compounds and the sample to be analyzed on the baseline
  4. Place the TLC plate in a closed container with solvent (ensuring the solvent doesn't cover the spots)
  5. Allow the solvent to travel up the plate, separating the components
  6. Remove the plate when the solvent nears the top and mark the solvent front

Vocabulary:

  • Retention factor (Rf): The ratio of the distance traveled by a component to the distance traveled by the solvent front

Example: If a component travels 4 cm and the solvent front travels 10 cm, the Rf value would be 4/10 = 0.4

Highlight: Reference compounds are crucial for identifying unknown components in the sample mixture.

Definition: The retention factor (Rf) is a key parameter in TLC, calculated as: Rf = distance traveled by component / distance traveled by solvent

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

View

Column Chromatography and Gas-Liquid Chromatography

This section covers the column chromatography setup instructions and principles of gas-liquid chromatography (GLC), two powerful separation techniques used in analytical chemistry.

Column Chromatography: • Uses a long vertical glass tube or burette as the column • Stationary phase: inert solid (e.g., powdered silica gel or alumina) • Mobile phase: liquid solvent

Column chromatography procedure:

  1. Carefully pack the column with the stationary phase
  2. Saturate the column with solvent
  3. Dissolve the sample mixture in solvent and add to the top of the column
  4. Add more solvent (eluent) to the column
  5. Allow gravity to pull the mobile phase through, or use pressure to speed up the process
  6. Collect separated components as they exit the column

Highlight: Proper column setup is crucial - cracks in the stationary phase can lead to separation issues.

Gas-Liquid Chromatography (GLC): • Used for gases, volatile liquids, and solids in vapor form • Stationary phase: non-volatile liquid on a solid support • Mobile phase: inert carrier gas (e.g., helium or nitrogen)

Gas-liquid chromatography principle:

  1. Inject the sample through a self-sealing disc
  2. Vaporize the sample
  3. Carrier gas moves sample molecules through the stationary phase
  4. Detector records retention times of separated components
  5. Chromatogram shows peaks representing each volatile compound

Vocabulary: Retention time - the time taken for a component to travel through the column

Definition: In GLC, retention time is used instead of Rf values to identify and quantify components.

Example: A larger peak on a GLC chromatogram indicates a greater quantity of that compound in the mixture.

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

View

Worked Example: Gas-Liquid Chromatography Analysis

This page presents a practical application of gas-liquid chromatography (GLC) through a worked example, demonstrating how to interpret chromatogram data.

The example shows the analysis of a compound by GLC, revealing the presence of four components: A, B, C, and D. The chromatogram displays the response over time, with peaks representing each component.

Questions and Answers:

  1. Which compound is present in the greatest quantity? Answer: Component D

Highlight: The larger the relative size of the peak, the greater the quantity of that substance present in the sample.

  1. Which compounds were present in equal amounts? Answer: Components B and C

Example: Equal peak sizes on a chromatogram indicate equal quantities of those components in the mixture.

  1. Which compound had the strongest interaction with the stationary phase? Answer: Component D

Vocabulary: Gas-liquid chromatography retention time - The time taken for a component to travel through the column, from injection to detection.

Highlight: The larger the retention time (i.e., the further to the right on the chromatogram), the greater the interaction of that component with the stationary phase.

This example illustrates key principles of interpreting GLC data:

  1. Peak size relates to quantity: Larger peaks indicate greater amounts of a component.
  2. Peak position indicates retention time: Components that interact more strongly with the stationary phase have longer retention times and appear further to the right on the chromatogram.
  3. Comparative analysis: By comparing peak sizes and positions, we can deduce relative quantities and interactions of different components in a mixture.

Understanding these principles is crucial for accurately analyzing complex mixtures using gas-liquid chromatography.

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

View

Fundamentals of Chromatography

Chromatography is a versatile analytical technique used to separate mixtures based on how their components interact differently with a stationary phase and a mobile phase. This section introduces the basic principles and three main types of chromatography.

The thin layer chromatography separation process relies on differences in how mixture components adsorb to a solid stationary phase and dissolve in a liquid mobile phase. The column chromatography procedure uses gravity or pressure to move the mobile phase through a vertical column of stationary phase material. Gas-liquid chromatography separates volatile compounds based on their partitioning between a liquid stationary phase and a gas mobile phase.

Definition: Chromatography is a separation technique that exploits differences in how components of a mixture interact with a stationary phase and a mobile phase.

Vocabulary:

  • Mobile phase: The fluid that carries the sample through the chromatography system
  • Stationary phase: The material that remains fixed in place during separation
  • Retention: How strongly a component is held back by the stationary phase

Highlight: The rate of separation in chromatography depends on two key factors:

  1. How components interact with the stationary phase
  2. How soluble they are in the mobile phase

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Subjects

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Chemistry

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Physical chemistry

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Kinetics

Fun Steps in Chromatography: TLC and Gas Chromatography for Kids

user profile picture

Aasiyah Rahman

@aasiyahrahman

·

44 Followers

Follow

Chromatography is a powerful technique for separating mixtures into their components. It relies on differences in how substances interact with a stationary phase and mobile phase. Thin layer chromatography separation process, gas-liquid chromatography retention time, and column chromatography setup instructions are key concepts in this field. The main types are thin-layer chromatography (TLC), column chromatography (CC), and gas-liquid chromatography (GLC), each with unique applications and procedures.

Key points:
• Chromatography separates mixtures based on component interactions with stationary and mobile phases
• TLC uses a solid silica plate and is useful for analyzing small samples
• Column chromatography employs a vertical tube filled with stationary phase material
• GLC is ideal for volatile compounds and uses retention time for identification
• Each method has specific setup procedures and analysis techniques

05/04/2023

91

 

12/13

 

Chemistry

1

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

Thin-Layer Chromatography (TLC)

Thin-layer chromatography (TLC) is a simple yet powerful technique for analyzing small samples through separation. This section covers the thin layer chromatography procedure and principles in detail.

The TLC stationary phase typically consists of a thin metal sheet coated with alumina (Al2O3) or silica (SiO2). Solute molecules adsorb onto this surface, with more strongly interacting components sticking more tightly. The mobile phase, usually a liquid solvent, flows over the stationary phase carrying the sample components.

To conduct a TLC analysis:

  1. Prepare a small volume of solute dissolved in solvent
  2. Draw a horizontal baseline in pencil near the bottom edge of the TLC plate
  3. Spot pure reference compounds and the sample to be analyzed on the baseline
  4. Place the TLC plate in a closed container with solvent (ensuring the solvent doesn't cover the spots)
  5. Allow the solvent to travel up the plate, separating the components
  6. Remove the plate when the solvent nears the top and mark the solvent front

Vocabulary:

  • Retention factor (Rf): The ratio of the distance traveled by a component to the distance traveled by the solvent front

Example: If a component travels 4 cm and the solvent front travels 10 cm, the Rf value would be 4/10 = 0.4

Highlight: Reference compounds are crucial for identifying unknown components in the sample mixture.

Definition: The retention factor (Rf) is a key parameter in TLC, calculated as: Rf = distance traveled by component / distance traveled by solvent

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

Column Chromatography and Gas-Liquid Chromatography

This section covers the column chromatography setup instructions and principles of gas-liquid chromatography (GLC), two powerful separation techniques used in analytical chemistry.

Column Chromatography: • Uses a long vertical glass tube or burette as the column • Stationary phase: inert solid (e.g., powdered silica gel or alumina) • Mobile phase: liquid solvent

Column chromatography procedure:

  1. Carefully pack the column with the stationary phase
  2. Saturate the column with solvent
  3. Dissolve the sample mixture in solvent and add to the top of the column
  4. Add more solvent (eluent) to the column
  5. Allow gravity to pull the mobile phase through, or use pressure to speed up the process
  6. Collect separated components as they exit the column

Highlight: Proper column setup is crucial - cracks in the stationary phase can lead to separation issues.

Gas-Liquid Chromatography (GLC): • Used for gases, volatile liquids, and solids in vapor form • Stationary phase: non-volatile liquid on a solid support • Mobile phase: inert carrier gas (e.g., helium or nitrogen)

Gas-liquid chromatography principle:

  1. Inject the sample through a self-sealing disc
  2. Vaporize the sample
  3. Carrier gas moves sample molecules through the stationary phase
  4. Detector records retention times of separated components
  5. Chromatogram shows peaks representing each volatile compound

Vocabulary: Retention time - the time taken for a component to travel through the column

Definition: In GLC, retention time is used instead of Rf values to identify and quantify components.

Example: A larger peak on a GLC chromatogram indicates a greater quantity of that compound in the mixture.

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

Worked Example: Gas-Liquid Chromatography Analysis

This page presents a practical application of gas-liquid chromatography (GLC) through a worked example, demonstrating how to interpret chromatogram data.

The example shows the analysis of a compound by GLC, revealing the presence of four components: A, B, C, and D. The chromatogram displays the response over time, with peaks representing each component.

Questions and Answers:

  1. Which compound is present in the greatest quantity? Answer: Component D

Highlight: The larger the relative size of the peak, the greater the quantity of that substance present in the sample.

  1. Which compounds were present in equal amounts? Answer: Components B and C

Example: Equal peak sizes on a chromatogram indicate equal quantities of those components in the mixture.

  1. Which compound had the strongest interaction with the stationary phase? Answer: Component D

Vocabulary: Gas-liquid chromatography retention time - The time taken for a component to travel through the column, from injection to detection.

Highlight: The larger the retention time (i.e., the further to the right on the chromatogram), the greater the interaction of that component with the stationary phase.

This example illustrates key principles of interpreting GLC data:

  1. Peak size relates to quantity: Larger peaks indicate greater amounts of a component.
  2. Peak position indicates retention time: Components that interact more strongly with the stationary phase have longer retention times and appear further to the right on the chromatogram.
  3. Comparative analysis: By comparing peak sizes and positions, we can deduce relative quantities and interactions of different components in a mixture.

Understanding these principles is crucial for accurately analyzing complex mixtures using gas-liquid chromatography.

Chromatography Separates mixtures 3 types Components in mixture dissolve in fluid (mobile phase) flow through another material (stationary p

Fundamentals of Chromatography

Chromatography is a versatile analytical technique used to separate mixtures based on how their components interact differently with a stationary phase and a mobile phase. This section introduces the basic principles and three main types of chromatography.

The thin layer chromatography separation process relies on differences in how mixture components adsorb to a solid stationary phase and dissolve in a liquid mobile phase. The column chromatography procedure uses gravity or pressure to move the mobile phase through a vertical column of stationary phase material. Gas-liquid chromatography separates volatile compounds based on their partitioning between a liquid stationary phase and a gas mobile phase.

Definition: Chromatography is a separation technique that exploits differences in how components of a mixture interact with a stationary phase and a mobile phase.

Vocabulary:

  • Mobile phase: The fluid that carries the sample through the chromatography system
  • Stationary phase: The material that remains fixed in place during separation
  • Retention: How strongly a component is held back by the stationary phase

Highlight: The rate of separation in chromatography depends on two key factors:

  1. How components interact with the stationary phase
  2. How soluble they are in the mobile phase

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Knowunity is the #1 education app in five European countries

Knowunity has been named a featured story on Apple and has regularly topped the app store charts in the education category in Germany, Italy, Poland, Switzerland, and the United Kingdom. Join Knowunity today and help millions of students around the world.

Ranked #1 Education App

Download in

Google Play

Download in

App Store

Knowunity is the #1 education app in five European countries

4.9+

Average app rating

15 M

Pupils love Knowunity

#1

In education app charts in 12 countries

950 K+

Students have uploaded notes

Still not convinced? See what other students are saying...

iOS User

I love this app so much, I also use it daily. I recommend Knowunity to everyone!!! I went from a D to an A with it :D

Philip, iOS User

The app is very simple and well designed. So far I have always found everything I was looking for :D

Lena, iOS user

I love this app ❤️ I actually use it every time I study.