How Temperature Affects Membrane Permeability
Temperature dramatically changes how membrane permeability works, and this has serious consequences for cell survival. Below 0°C, phospholipids barely move, creating a rigid structure where ice crystals can punch holes through the membrane - definitely not ideal for keeping your cell contents inside!
Between 0-45°C, things get interesting. As temperature rises, phospholipids move more freely, making the membrane increasingly fluid and permeable. This is why the beetroot practical works - higher temperatures release more red pigment because the membrane becomes leakier.
Above 45°C, it's game over. The bilayer melts, proteins lose their shape, and the membrane basically falls apart. Water expands inside the cell, putting massive pressure on the already damaged membrane structure.
Practical Tip: In the beetroot experiment, the deeper the red colour in your test tube, the more permeable the membrane has become at that temperature.
Solvents like ethanol also increase permeability by dissolving the lipids that hold the membrane together. This is why alcohol can be toxic to cells - it literally breaks down their protective barriers.