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How to Use a Microscope Step by Step and Differences Between Prokaryotic and Eukaryotic Cells

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How to Use a Microscope Step by Step and Differences Between Prokaryotic and Eukaryotic Cells
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emily

@onlyemss

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This comprehensive guide covers key topics in cell biology, including microscopy techniques, cell structures, and cellular processes. It provides detailed explanations of how to use microscope step-by-step, comparisons between prokaryotic and eukaryotic cells, and adaptations of specialized cell types.

Key points:

  • Advantages and disadvantages of light and electron microscopes
  • Steps for preparing and viewing specimens under a microscope
  • Structures and functions of cell organelles
  • Differences between prokaryotic and eukaryotic cells
  • Adaptations of specialized cell types like nerve, muscle, and sperm cells
  • Cell cycle stages and importance of mitosis
  • Aseptic techniques for culturing bacteria

28/12/2023

320

Cell Division and Aseptic Techniques

This section covers the cell cycle, mitosis, and proper laboratory techniques for culturing bacteria.

The three main stages of the cell cycle are described:

  1. Interphase - Growth and DNA replication
  2. Mitosis - Nuclear division
  3. Cytokinesis - Cytoplasmic division

Definition: Mitosis - The process of nuclear division resulting in two genetically identical daughter nuclei.

The importance of mitosis for growth, repair, and asexual reproduction is explained.

Proper aseptic techniques for preparing uncontaminated bacterial cultures are outlined, including sterilizing equipment and using a Bunsen burner flame.

Highlight: Aseptic technique is crucial for preventing contamination when culturing microorganisms in the laboratory.

The guide also defines key terms like chromosomes, genes, and DNA. Understanding these fundamental concepts is essential for studying cell biology and genetics.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Microscopy Techniques and Cell Structures

This section covers the advantages and disadvantages of light and electron microscopes, as well as key cellular structures and differences between cell types.

Highlight: Light microscopes are relatively inexpensive and portable, but have limited magnification up to about 2000x. Electron microscopes offer much higher magnification up to 2,000,000x and better resolution, but are very expensive and require controlled environments.

The 10 steps in using microscope are outlined, including preparing slides, adjusting focus, and viewing specimens at increasing magnifications.

Example: To prepare an onion cell slide:

  1. Peel epidermal tissue from an onion
  2. Place tissue on slide with a drop of water
  3. Add iodine stain
  4. Carefully place coverslip to avoid air bubbles

Key differences between prokaryotic and eukaryotic cells are explained, such as the presence of membrane-bound organelles in eukaryotes. The guide also describes how various specialized cell types are adapted for their functions.

Vocabulary: Differentiation - The process by which cells become specialized for particular functions as an organism develops.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

Specialized Cell Types and Their Adaptations

This section examines how various cell types are adapted for their specific functions in the body.

Nerve cells have unique adaptations including:

  • Long axons to transmit nerve impulses over distances
  • Many dendrites to form connections with other neurons
  • Synapses for passing signals between cells
  • Myelin sheath for insulation and faster signal conduction

Muscle cells are adapted with:

  • Special contractile proteins that slide past each other
  • Many mitochondria to produce energy for movement
  • Glycogen stores for cellular respiration

Example: Sperm cells have a long tail for motility, many mitochondria for energy production, and an enzyme-filled acrosome for penetrating the egg.

Plant cells like root hair cells and xylem/phloem cells have adaptations for water/nutrient uptake and transport. Photosynthetic cells contain chloroplasts and are positioned to maximize light absorption.

Understanding these specialized adaptations helps explain how different cell types carry out their vital roles within organisms.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

Prokaryotic vs Eukaryotic Cells

This section compares and contrasts prokaryotic and eukaryotic cell types.

Key differences between prokaryotic and eukaryotic cells include:

  1. Size - Prokaryotes are generally smaller
  2. Organelles - Eukaryotes have membrane-bound organelles like mitochondria and nucleus
  3. DNA arrangement - Prokaryotes have a single DNA loop in the cytoplasm, while eukaryotes have DNA organized in chromosomes within a nucleus
  4. Presence of plasmids in prokaryotes

Highlight: The 10 differences between prokaryotic and eukaryotic cells include structural features as well as genetic organization.

Some similarities between prokaryotic and eukaryotic cells are also noted, such as the presence of cytoplasm, a cell membrane, and genetic material.

Understanding these fundamental differences is crucial for biology students to grasp the diversity of cell types.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

Microscopy Techniques in Detail

This section provides an in-depth look at how to use microscope in laboratory settings, comparing light and electron microscopy.

Light microscopes:

  • Magnification up to ~2000x
  • Resolution around 200 nm
  • Useful for viewing stained cells and observing cell division

Electron microscopes:

  • Magnification up to ~2,000,000x
  • Resolution as low as 0.2 nm for transmission electron microscopes
  • Can examine detailed subcellular structures

Vocabulary: Resolution - The ability to distinguish between two separate points or objects.

The guide outlines the steps for how to focus a microscope step by step, emphasizing the importance of starting with the lowest magnification and gradually increasing.

Example: When focusing, first use the coarse adjustment to bring the specimen into view, then use the fine adjustment for crisp detail.

Understanding the capabilities and limitations of different microscopy techniques is essential for choosing the appropriate method for specific research applications.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

View

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Can't find what you're looking for? Explore other subjects.

Knowunity is the #1 education app in five European countries

Knowunity has been named a featured story on Apple and has regularly topped the app store charts in the education category in Germany, Italy, Poland, Switzerland, and the United Kingdom. Join Knowunity today and help millions of students around the world.

Ranked #1 Education App

Download in

Google Play

Download in

App Store

Knowunity is the #1 education app in five European countries

4.9+

Average app rating

13 M

Pupils love Knowunity

#1

In education app charts in 12 countries

950 K+

Students have uploaded notes

Still not convinced? See what other students are saying...

iOS User

I love this app so much, I also use it daily. I recommend Knowunity to everyone!!! I went from a D to an A with it :D

Philip, iOS User

The app is very simple and well designed. So far I have always found everything I was looking for :D

Lena, iOS user

I love this app ❤️ I actually use it every time I study.

View

How to Use a Microscope Step by Step and Differences Between Prokaryotic and Eukaryotic Cells
user profile picture

emily

@onlyemss

·

3 Followers

Follow

How to Use a Microscope Step by Step and Differences Between Prokaryotic and Eukaryotic Cells

This comprehensive guide covers key topics in cell biology, including microscopy techniques, cell structures, and cellular processes. It provides detailed explanations of how to use microscope step-by-step, comparisons between prokaryotic and eukaryotic cells, and adaptations of specialized cell types.

Key points:

  • Advantages and disadvantages of light and electron microscopes
  • Steps for preparing and viewing specimens under a microscope
  • Structures and functions of cell organelles
  • Differences between prokaryotic and eukaryotic cells
  • Adaptations of specialized cell types like nerve, muscle, and sperm cells
  • Cell cycle stages and importance of mitosis
  • Aseptic techniques for culturing bacteria

28/12/2023

320

Cell Division and Aseptic Techniques

This section covers the cell cycle, mitosis, and proper laboratory techniques for culturing bacteria.

The three main stages of the cell cycle are described:

  1. Interphase - Growth and DNA replication
  2. Mitosis - Nuclear division
  3. Cytokinesis - Cytoplasmic division

Definition: Mitosis - The process of nuclear division resulting in two genetically identical daughter nuclei.

The importance of mitosis for growth, repair, and asexual reproduction is explained.

Proper aseptic techniques for preparing uncontaminated bacterial cultures are outlined, including sterilizing equipment and using a Bunsen burner flame.

Highlight: Aseptic technique is crucial for preventing contamination when culturing microorganisms in the laboratory.

The guide also defines key terms like chromosomes, genes, and DNA. Understanding these fundamental concepts is essential for studying cell biology and genetics.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

Sign up to get unlimited access to thousands of study materials. It's free!

Access to all documents

Join milions of students

Improve your grades

By signing up you accept Terms of Service and Privacy Policy

Microscopy Techniques and Cell Structures

This section covers the advantages and disadvantages of light and electron microscopes, as well as key cellular structures and differences between cell types.

Highlight: Light microscopes are relatively inexpensive and portable, but have limited magnification up to about 2000x. Electron microscopes offer much higher magnification up to 2,000,000x and better resolution, but are very expensive and require controlled environments.

The 10 steps in using microscope are outlined, including preparing slides, adjusting focus, and viewing specimens at increasing magnifications.

Example: To prepare an onion cell slide:

  1. Peel epidermal tissue from an onion
  2. Place tissue on slide with a drop of water
  3. Add iodine stain
  4. Carefully place coverslip to avoid air bubbles

Key differences between prokaryotic and eukaryotic cells are explained, such as the presence of membrane-bound organelles in eukaryotes. The guide also describes how various specialized cell types are adapted for their functions.

Vocabulary: Differentiation - The process by which cells become specialized for particular functions as an organism develops.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

Sign up to get unlimited access to thousands of study materials. It's free!

Access to all documents

Join milions of students

Improve your grades

By signing up you accept Terms of Service and Privacy Policy

Specialized Cell Types and Their Adaptations

This section examines how various cell types are adapted for their specific functions in the body.

Nerve cells have unique adaptations including:

  • Long axons to transmit nerve impulses over distances
  • Many dendrites to form connections with other neurons
  • Synapses for passing signals between cells
  • Myelin sheath for insulation and faster signal conduction

Muscle cells are adapted with:

  • Special contractile proteins that slide past each other
  • Many mitochondria to produce energy for movement
  • Glycogen stores for cellular respiration

Example: Sperm cells have a long tail for motility, many mitochondria for energy production, and an enzyme-filled acrosome for penetrating the egg.

Plant cells like root hair cells and xylem/phloem cells have adaptations for water/nutrient uptake and transport. Photosynthetic cells contain chloroplasts and are positioned to maximize light absorption.

Understanding these specialized adaptations helps explain how different cell types carry out their vital roles within organisms.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

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Access to all documents

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Prokaryotic vs Eukaryotic Cells

This section compares and contrasts prokaryotic and eukaryotic cell types.

Key differences between prokaryotic and eukaryotic cells include:

  1. Size - Prokaryotes are generally smaller
  2. Organelles - Eukaryotes have membrane-bound organelles like mitochondria and nucleus
  3. DNA arrangement - Prokaryotes have a single DNA loop in the cytoplasm, while eukaryotes have DNA organized in chromosomes within a nucleus
  4. Presence of plasmids in prokaryotes

Highlight: The 10 differences between prokaryotic and eukaryotic cells include structural features as well as genetic organization.

Some similarities between prokaryotic and eukaryotic cells are also noted, such as the presence of cytoplasm, a cell membrane, and genetic material.

Understanding these fundamental differences is crucial for biology students to grasp the diversity of cell types.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

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Access to all documents

Join milions of students

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Microscopy Techniques in Detail

This section provides an in-depth look at how to use microscope in laboratory settings, comparing light and electron microscopy.

Light microscopes:

  • Magnification up to ~2000x
  • Resolution around 200 nm
  • Useful for viewing stained cells and observing cell division

Electron microscopes:

  • Magnification up to ~2,000,000x
  • Resolution as low as 0.2 nm for transmission electron microscopes
  • Can examine detailed subcellular structures

Vocabulary: Resolution - The ability to distinguish between two separate points or objects.

The guide outlines the steps for how to focus a microscope step by step, emphasizing the importance of starting with the lowest magnification and gradually increasing.

Example: When focusing, first use the coarse adjustment to bring the specimen into view, then use the fine adjustment for crisp detail.

Understanding the capabilities and limitations of different microscopy techniques is essential for choosing the appropriate method for specific research applications.

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

Sign up to get unlimited access to thousands of study materials. It's free!

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By signing up you accept Terms of Service and Privacy Policy

CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
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microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
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microscopes
Describe the
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electron microscope

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CELL BIOLOGY
Describe the
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microscopes
Describe the
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disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
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disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
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microscopes
Describe the
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disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
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Describe the
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electron microscope

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Describe the
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electron microscope

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Describe the
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electron microscope

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CELL BIOLOGY
Describe the
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electron microscope

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electron microscope

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electron microscope

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electron microscope

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electron microscope

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Describe the
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Describe the
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electron microscope

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Describe the
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electron microscope

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

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CELL BIOLOGY
Describe the
advantages and
disadvantages of light
microscopes
Describe the
advantages and
disadvantages of
electron microscope

Register

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Access to all documents

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