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150

15 Oct 2022

4 pages

Understanding Enzymes: Active Sites, Induced Fit Model, and Temperature Effects - AQA Biology

user profile picture

Azelya Comak

@azelyacomak_

Enzymes are biological catalysts that speed up chemical reactions in... Show more

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp

Factors Affecting Enzyme Activity

This page discusses various factors that influence enzyme activity, including temperature, pH, and substrate concentration.

Temperature Effects

Enzymes allow reactions to occur at lower temperatures than they would without the enzyme present. They do this by:

  1. Holding substrates close together, reducing repulsion and facilitating bonding.
  2. Putting strain on substrate bonds, making them easier to break.

Highlight: Understanding the effect of temperature on enzyme activity is crucial for AQA GCSE Biology enzymes exam questions.

However, excessive heat can lead to enzyme denaturation:

Definition: Denaturing occurs when hydrogen bonds break and the tertiary structure of the enzyme unfolds, rendering the active site non-complementary to the substrate.

pH Effects

The page includes graphs showing the relationship between enzyme activity and pH:

  1. A bell-shaped curve illustrating the optimum pH for enzyme activity.
  2. A logarithmic scale graph showing enzyme activity across a wider pH range.

Example: The effect of pH on enzyme activity can be demonstrated through practical experiments, often featured in AQA A Level Biology enzymes exam questions.

Enzyme and Substrate Concentration

Graphs illustrate how enzyme and substrate concentrations affect reaction rates:

  1. Enzyme concentration: Initially, increasing enzyme concentration increases reaction rate linearly until substrate becomes a limiting factor.
  2. Substrate concentration: Reaction rate increases with substrate concentration until enzyme saturation occurs.

Vocabulary: A limiting factor is a variable that constrains the rate of a reaction when other factors are in excess.

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp

Enzyme Inhibitors

This page focuses on enzyme inhibitors, which are substances that reduce enzyme activity.

Types of Inhibitors

There are two main types of reversible inhibitors:

  1. Competitive Inhibitors Have a similar shape to the substrate Can occupy the enzyme's active site Compete with the substrate for binding Inhibition depends on relative concentrations of substrate and inhibitor
  2. Non-competitive Inhibitors Attach to enzymes away from the active site Alter the shape of the active site Not in direct competition with the substrate Adding more substrate does not decrease their effect

Highlight: Understanding enzyme inhibitors is crucial for AQA A Level Biology students, as it's often featured in exam questions and practical assessments.

Key Points About Enzymes

The page concludes with a summary of essential enzyme characteristics:

  • Enzymes are biological catalysts made of globular proteins
  • They lower activation energy without being consumed in the reaction
  • Enzymes bind to substrates via their complementary active site
  • This binding forms an enzyme-substrate complex

Example: The induced fit model vs lock and key comparison is a common topic in A Level Biology courses, illustrating different theories of enzyme-substrate interaction.

Vocabulary: The enzyme-substrate complex is the temporary structure formed when an enzyme binds to its specific substrate during a reaction.

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp

Enzyme Structure and Function

Enzymes are biological catalysts made of globular proteins that play a crucial role in facilitating biochemical reactions. They maintain their highly specific shape through chemical bonds such as hydrogen bonds, ionic bonds, and disulphide bridges.

Definition: Enzymes are biological catalysts that speed up chemical reactions without being permanently changed in the process.

The active site of an enzyme is formed by a small number of amino acids in the polypeptide chains, but the overall shape is determined by the entire amino acid sequence and the bonds formed during protein folding.

Highlight: Enzymes can be found both intracellularly and extracellularly, participating in anabolic buildingbuilding and catabolic breakingbreaking reactions.

Collision Theory and Enzyme Action

Collision theory explains how enzymes work at the molecular level:

  1. Molecules move randomly due to kinetic energy.
  2. Reactions occur when molecules collide with sufficient energy to overcome the activation energy barrier.
  3. Enzymes lower this activation energy, making reactions more likely to occur.

Example: In the induced fit model of enzyme action, the enzyme's shape changes slightly to accommodate the substrate, forming an enzyme-substrate complex. This model is crucial for understanding enzyme active site interaction in AQA Biology GCSE and A-level.

Enzyme Action Diagram

The document includes a diagram illustrating enzyme action, comparing the energy profiles of reactions with and without enzymes. It shows how enzymes lower the activation energy required for a reaction to occur.

Vocabulary: Activation energy is the minimum energy required for a chemical reaction to take place.

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp


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4.9/5

App Store

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The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because it’s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user

The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because it’s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user

 

Biology

150

15 Oct 2022

4 pages

Understanding Enzymes: Active Sites, Induced Fit Model, and Temperature Effects - AQA Biology

user profile picture

Azelya Comak

@azelyacomak_

Enzymes are biological catalysts that speed up chemical reactions in living organisms without being consumed in the process. They play a vital role in essential life processes.

The induced fit model of enzyme actionexplains how enzymes work through a... Show more

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp

Sign up to see the contentIt's free!

Access to all documents

Improve your grades

Join milions of students

By signing up you accept Terms of Service and Privacy Policy

Factors Affecting Enzyme Activity

This page discusses various factors that influence enzyme activity, including temperature, pH, and substrate concentration.

Temperature Effects

Enzymes allow reactions to occur at lower temperatures than they would without the enzyme present. They do this by:

  1. Holding substrates close together, reducing repulsion and facilitating bonding.
  2. Putting strain on substrate bonds, making them easier to break.

Highlight: Understanding the effect of temperature on enzyme activity is crucial for AQA GCSE Biology enzymes exam questions.

However, excessive heat can lead to enzyme denaturation:

Definition: Denaturing occurs when hydrogen bonds break and the tertiary structure of the enzyme unfolds, rendering the active site non-complementary to the substrate.

pH Effects

The page includes graphs showing the relationship between enzyme activity and pH:

  1. A bell-shaped curve illustrating the optimum pH for enzyme activity.
  2. A logarithmic scale graph showing enzyme activity across a wider pH range.

Example: The effect of pH on enzyme activity can be demonstrated through practical experiments, often featured in AQA A Level Biology enzymes exam questions.

Enzyme and Substrate Concentration

Graphs illustrate how enzyme and substrate concentrations affect reaction rates:

  1. Enzyme concentration: Initially, increasing enzyme concentration increases reaction rate linearly until substrate becomes a limiting factor.
  2. Substrate concentration: Reaction rate increases with substrate concentration until enzyme saturation occurs.

Vocabulary: A limiting factor is a variable that constrains the rate of a reaction when other factors are in excess.

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp

Sign up to see the contentIt's free!

Access to all documents

Improve your grades

Join milions of students

By signing up you accept Terms of Service and Privacy Policy

Enzyme Inhibitors

This page focuses on enzyme inhibitors, which are substances that reduce enzyme activity.

Types of Inhibitors

There are two main types of reversible inhibitors:

  1. Competitive Inhibitors Have a similar shape to the substrate Can occupy the enzyme's active site Compete with the substrate for binding Inhibition depends on relative concentrations of substrate and inhibitor
  2. Non-competitive Inhibitors Attach to enzymes away from the active site Alter the shape of the active site Not in direct competition with the substrate Adding more substrate does not decrease their effect

Highlight: Understanding enzyme inhibitors is crucial for AQA A Level Biology students, as it's often featured in exam questions and practical assessments.

Key Points About Enzymes

The page concludes with a summary of essential enzyme characteristics:

  • Enzymes are biological catalysts made of globular proteins
  • They lower activation energy without being consumed in the reaction
  • Enzymes bind to substrates via their complementary active site
  • This binding forms an enzyme-substrate complex

Example: The induced fit model vs lock and key comparison is a common topic in A Level Biology courses, illustrating different theories of enzyme-substrate interaction.

Vocabulary: The enzyme-substrate complex is the temporary structure formed when an enzyme binds to its specific substrate during a reaction.

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp

Sign up to see the contentIt's free!

Access to all documents

Improve your grades

Join milions of students

By signing up you accept Terms of Service and Privacy Policy

Enzyme Structure and Function

Enzymes are biological catalysts made of globular proteins that play a crucial role in facilitating biochemical reactions. They maintain their highly specific shape through chemical bonds such as hydrogen bonds, ionic bonds, and disulphide bridges.

Definition: Enzymes are biological catalysts that speed up chemical reactions without being permanently changed in the process.

The active site of an enzyme is formed by a small number of amino acids in the polypeptide chains, but the overall shape is determined by the entire amino acid sequence and the bonds formed during protein folding.

Highlight: Enzymes can be found both intracellularly and extracellularly, participating in anabolic buildingbuilding and catabolic breakingbreaking reactions.

Collision Theory and Enzyme Action

Collision theory explains how enzymes work at the molecular level:

  1. Molecules move randomly due to kinetic energy.
  2. Reactions occur when molecules collide with sufficient energy to overcome the activation energy barrier.
  3. Enzymes lower this activation energy, making reactions more likely to occur.

Example: In the induced fit model of enzyme action, the enzyme's shape changes slightly to accommodate the substrate, forming an enzyme-substrate complex. This model is crucial for understanding enzyme active site interaction in AQA Biology GCSE and A-level.

Enzyme Action Diagram

The document includes a diagram illustrating enzyme action, comparing the energy profiles of reactions with and without enzymes. It shows how enzymes lower the activation energy required for a reaction to occur.

Vocabulary: Activation energy is the minimum energy required for a chemical reaction to take place.

• Enzymes are biological catalysts
• Made of globular proteins
• Highly specific shape maintained by chemical bonds (hydrogen, ionic, disulp

Sign up to see the contentIt's free!

Access to all documents

Improve your grades

Join milions of students

By signing up you accept Terms of Service and Privacy Policy

Can't find what you're looking for? Explore other subjects.

Students love us — and so will you.

4.9/5

App Store

4.8/5

Google Play

The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because it’s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user

The app is very easy to use and well designed. I have found everything I was looking for so far and have been able to learn a lot from the presentations! I will definitely use the app for a class assignment! And of course it also helps a lot as an inspiration.

Stefan S

iOS user

This app is really great. There are so many study notes and help [...]. My problem subject is French, for example, and the app has so many options for help. Thanks to this app, I have improved my French. I would recommend it to anyone.

Samantha Klich

Android user

Wow, I am really amazed. I just tried the app because I've seen it advertised many times and was absolutely stunned. This app is THE HELP you want for school and above all, it offers so many things, such as workouts and fact sheets, which have been VERY helpful to me personally.

Anna

iOS user

Best app on earth! no words because it’s too good

Thomas R

iOS user

Just amazing. Let's me revise 10x better, this app is a quick 10/10. I highly recommend it to anyone. I can watch and search for notes. I can save them in the subject folder. I can revise it any time when I come back. If you haven't tried this app, you're really missing out.

Basil

Android user

This app has made me feel so much more confident in my exam prep, not only through boosting my own self confidence through the features that allow you to connect with others and feel less alone, but also through the way the app itself is centred around making you feel better. It is easy to navigate, fun to use, and helpful to anyone struggling in absolutely any way.

David K

iOS user

The app's just great! All I have to do is enter the topic in the search bar and I get the response real fast. I don't have to watch 10 YouTube videos to understand something, so I'm saving my time. Highly recommended!

Sudenaz Ocak

Android user

In school I was really bad at maths but thanks to the app, I am doing better now. I am so grateful that you made the app.

Greenlight Bonnie

Android user

very reliable app to help and grow your ideas of Maths, English and other related topics in your works. please use this app if your struggling in areas, this app is key for that. wish I'd of done a review before. and it's also free so don't worry about that.

Rohan U

Android user

I know a lot of apps use fake accounts to boost their reviews but this app deserves it all. Originally I was getting 4 in my English exams and this time I got a grade 7. I didn’t even know about this app three days until the exam and it has helped A LOT. Please actually trust me and use it as I’m sure you too will see developments.

Xander S

iOS user

THE QUIZES AND FLASHCARDS ARE SO USEFUL AND I LOVE THE SCHOOLGPT. IT ALSO IS LITREALLY LIKE CHATGPT BUT SMARTER!! HELPED ME WITH MY MASCARA PROBLEMS TOO!! AS WELL AS MY REAL SUBJECTS ! DUHHH 😍😁😲🤑💗✨🎀😮

Elisha

iOS user

This apps acc the goat. I find revision so boring but this app makes it so easy to organize it all and then you can ask the freeeee ai to test yourself so good and you can easily upload your own stuff. highly recommend as someone taking mocks now

Paul T

iOS user