- Semiconservative DNA Replication is the process of creating two daughter cells with one original and one new DNA strand.
- 5 Stages of DNA Replication: unwinding and unzipping, lining up nucleotides, joining bases and nucleotides, forming the 'Sugar Phosphate backbone', and rewinding to form a double helix structure.
- Leading and Lagging Strands: Leading strand replicates continuously, while the lagging strand replicates discontinuously in fragments before being joined together.
- Polymerase Chain Reaction (PCR) is used to amplify DNA samples and requires a DNA template, primers, nucleotides, and heat-tolerant DNA polymerase.
- Principle of PCR: Involves heating to break hydrogen bonds, cooling to allow primers to anneal, and heating again for DNA polymerase to add nucleotides.
Understanding the steps and principles of DNA replication process step by step and PCR is crucial for biology and genetic research.
